Locus Details

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MENotes Database ID 33495
Taxon Silene latifolia (Caryophyllaceae)
Locus Name SlX1 ( All species amplified... )
Marker Type microsatellite
Genome Not Provided
Original Species Not Provided, Genbank ID AY084036.1
Publication Reference Molecular Ecology Resources (2009) 8:1274-1276
Contact Information Roman Hobza
hobza@ibp.cz
Forward Primer (5'–3') CGAGGCATCTATCTGGGACA
Reverse Primer (5'–3') CGGCAACTAAATGGTCAGATG
Annealing Temperature 55°C
Number of Alleles 2
Size Range 387
Expected Heterozygosity Not Provided
Observed Heterozygosity Not Provided
Notes After denaturation at 94°C for 2 min, cycling was performed for 35 cycles under the following conditions: 30 s at 94°C, 45 s at 55°C and 45 s at 72°C and a final extension step for 5 min at 72°C.